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MicroRNAs as Tools in Biopharmaceutical Production

  • Erscheinungsdatum: 15.11.2012
  • Verlag: Springer-Verlag
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MicroRNAs as Tools in Biopharmaceutical Production

Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Produktinformationen

    Format: PDF
    Kopierschutz: AdobeDRM
    Seitenzahl: 126
    Erscheinungsdatum: 15.11.2012
    Sprache: Englisch
    ISBN: 9789400751286
    Verlag: Springer-Verlag
    Größe: 1471kBytes
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