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MALDI MS A Practical Guide to Instrumentation, Methods and Applications

  • Erscheinungsdatum: 08.10.2013
  • Verlag: Wiley-Blackwell
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This authoritative book on MALDI MS, now finally available in its second edition and edited by one of its inventors, gives an in-depth description of the many different applications, along with a detailed discussion of the technology itself. Thoroughly updated and expanded, with contributions from key players in the field, this unique book provides a comprehensive overview of MALDI MS along with its possibilities and limitations. The initial chapters deal with the technology and the instrumental setup, followed by chapters on the use of MALDI MS in protein research (including proteomics), genomics, glycomics and lipidomics. The possibilities if MALDI-MS for the analysis of polymers and small molecules are also covered in separate chapters, while new to this edition is a section devoted to the interplay of MALDI MS and bioinformatics. A much-needed practical and educational asset for individuals, academic institutions and companies in the field of bioanalytics. Franz Hillenkamp is Professor Emeritus at the University of Munster, Germany. He holds a MS degree in electrical engineering from Purdue University, USA, and a PhD from the Technical University of Munich, Germany. Before he was appointed Professor of Biophysics and Medical Physics at Munster in 1986, he held a professorship at the University of Frankfurt, Germany. During the 1980s, he developed the now world famous MALDI technique that was later on shown to be highly useful for the analysis of biomolecules. For his ground-breaking work on mass spectrometry methods, Professor Hillenkamp has received numerous awards, among them the Thompson Medal of the Mass Spectrometry Society, the Fresenius Medal of the German Chemical Society, and the Bergman Medal of the Swedish Chemical Society. Jasna Peter-Katalinic is Professor at the University of Rijeka, Croatia, and former Associate Professor of Biophysics at the University of Munster, Germany. She was born and educated in Zagreb, Croatia, and obtained her PhD in chemistry at the University of Zurich, Switzerland. After the postdoc time at the Texas A+M University, USA, she obtained the habilitation in physiological chemistry from the University of Bonn, Germany. She pioneered the introduction of modern mass spectrometric methods to structural glycobiology/glycomics, as described in more than 250 publications. Her current interests are in the Human Glycoproteome Initiative and Nanobioanalytics. She was the first recipient of the Life Science Award from the German Society of Mass Spectrometry in 2002.


    Format: ePUB
    Kopierschutz: AdobeDRM
    Seitenzahl: 430
    Erscheinungsdatum: 08.10.2013
    Sprache: Englisch
    ISBN: 9783527673742
    Verlag: Wiley-Blackwell
    Größe: 12405 kBytes
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The MALDI Process and Method

Franz Hillenkamp, Thorsten W. Jaskolla, and Michael Karas
1.1 Introduction

Matrix-assisted laser desorption/ionization ( MALDI ) is one of the two "soft" ionization techniques besides electrospray ionization ( ESI ) which allow for the sensitive detection of large, nonvolatile and labile molecules by mass spectrometry. Over the past 27 years, MALDI has developed into an indispensable tool in analytical chemistry, and in analytical biochemistry in particular. In this chapter, the reader will be introduced to the technology as it stands now, and some of the underlying physical and chemical mechanisms as far as they have been investigated and clarified to date will be discussed.

Attention will also be focused on the central issues of MALDI, that are necessary for the user to understand for the efficient application of this technique. As an in-depth discussion of these topics is beyond the scope of this chapter, the reader is referred to recent reviews [1-4]. Details of the current state of instrumentation, including lasers and their coupling to mass spectrometers, will be presented in Chapter 2 .

As with most new technologies, MALDI came as rather a surprise even to the experts in the field on the one hand, but also evolved from a diversity of prior art and knowledge on the other hand. The original notion had been that (bio)molecules with masses in excess of about 500-1000 Da could not be isolated out of their natural (e.g., aqueous) environment, and even less be charged for an analysis in the vacuum of a mass spectrometer without excessive and unspecific fragmentation. During the late 1960s, however, Beckey introduced field desorption ( FD ), the first technique to open a small road into the territory of mass spectrometry ( MS ) of bioorganic molecules [5]. Next came secondary ion mass spectrometry ( SIMS ), and in particular static SIMS, as introduced by A. Benninghoven in 1975 [6]. This development was taken a step further by M. Barber in 1981, with the bombardment of organic compounds dissolved in glycerol with high-energy atoms, which Barber coined fast atom bombardment ( FAB ). It was in this context, and in conjunction with the first attempts to desorb organic molecules with laser irradiation, that the concept of a "matrix" as a means of facilitating desorption and enhancing ion yield was born [7]. The principle of desorption by the bombardment of organic samples with the fission products of the 252Cf nuclear decay, later termed plasma desorption ( PD ), was first described by R. Macfarlane in 1974 [8]. Subsequently, the groups of Sundqvist and Roepstoff greatly improved the analytical potential of this technique by the addition of nitrocellulose, which not only cleaned up the sample but was also suspected of functioning as a signal-enhancing matrix [9].

The first attempts at using laser radiation to generate ions for a mass spectrometric analysis were reported only a few years after the invention of the laser [10, 11]. Vastola and Pirone had already demonstrated the possibility of recording the spectra of organic compounds with a time-of-flight ( TOF ) mass spectrometer. Subsequently, several groups continued to pursue this line of research, mainly R. Cotter at Johns Hopkins University in the USA and P. Kistemaker at the FOM Institute in Amsterdam, the Netherlands. Indeed, for a number of years the Amsterdam group held the high-mass record for a bioorganic analyte with a spectrum of underivatized digitonin at mass 1251 Da ([M + Na]+), desorbed with a CO2-laser at a wavelength of 10.6 mim in the far infrared ( IR ) [12].

Independently of, and paral

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